Identification of foreign bodies
12 June 2023
Shantelle Dandy, Section Lead - Microscopy
Rapid and accurate identification of physical contaminants (foreign body material) found in food is essential in
identifying the source of contamination and implementing measures to handle the incident and prevent reoccurrence. Pinpointing the stage in
the food chain at which contamination took place can require skilled detective work. Information may be required on: country of origin;
whether contamination occurred before or after processing, in the factory or during storage and distribution, at retail point of sale or
whether contamination occurred in the consumer’s home. An assessment may also be needed on the likelihood of the problem being restricted to
one product or batch, or whether it is more widespread.
Ultimately a company or enforcement authority has to decide whether they are handling a safety issue with product
recall implications, or a quality issue related to consumer perception or fair trading.
A useful working definition of a foreign body is “an object which can be seen by the unaided eye or felt in the mouth,
and which the consumer perceives as being alien to the food”. This will therefore include not only items that are obviously foreign, such as
insects, stone or glass, but also materials that are connected with the food, such as bits of stalk or apple core and burnt product. Foreign
bodies may get into the food product at any point between growth of the raw materials and the consumer’s mouth.
A whole range of analytical techniques are used for identification of foreign bodies: microscopy techniques such as
light microscopy, scanning electron microscopy, infra-red spectroscopy and gas chromatography, and conventional wet chemistry methods.
Light microscopy is used routinely for the examination of the physical structure / morphology of samples, and this
can provide useful evidence in their identification. It can help identify the type of biological material, such as a plant stem or bone.
Scanning Electron Microscopy and X-ray Microanalysis
The Scanning Electron Microscope (SEM) gives pseudo-three dimensional images with higher magnification and greater
depth of focus than the light microscope. SEM is an invaluable tool for the rapid examination of the three-dimensional structure of many
More detailed microanalysis of SEM samples may be carried out using an energy-dispersive X-ray microanalyser. The
energies of the X-rays given off by a sample irradiated with an electron beam are characteristic of the elements present in the sample, and
so the X-ray microanalyser can be used to give a non-destructive elemental analysis of the sample. The primary application of the method in
foreign body identifications is in the analysis of inorganic objects such as glass, metal and stone; it can also rapidly identify samples
erroneously reported as glass such as struvite (magnesium ammonium phosphate), salt and silica minerals.
Identification strategies and aims
The two primary questions that the analyst is seeking to answer are: what is the foreign body? and how did it get
into the product? The latter is often very difficult to achieve. It is important not to destroy evidence that could answer one question
whilst answering the other.
The initial approach to the examination of a foreign body should be made with great care, to avoid disturbing the
sample and possibly destroying vital evidence. Notes should be made, possibly using a light stereomicroscope, and photographs may be taken.
If the sample is still embedded in the food product, consider how it might have become embedded before removing it. If fragments of food
material are still attached to the surface, these can be examined to see if they support the reported circumstances of the discovery. A major
question that most clients would like answering is: was the foreign body processed with the product? Unfortunately, this is often very
difficult to determine.
Some foreign body samples will be so familiar as to be readily identifiable on sight. They may be intact manufactured
items, and the shape of such objects is particularly important in their recognition. These may include nuts, bolts and screws, staples,
matchsticks, buttons and other everyday objects. In these cases the more important information will come from surface deposits, scratches or
staining which may provide clues as to how and when the object got into the food. Also important is elemental composition. This could provide
information on whether a sample would have been picked up by an online detector.
Types of foreign bodies
Animal tissue may include pieces of malformed food material, pieces of the animal which should have been excluded
from the food, such as skin, bone, cartilage, etc, and animal products such as faeces. Animal tissue may sometimes change in appearance as a
result of processing or drying, so that, for example, dried muscle tissue can look very different from fresh meat. Spots of blood on the
surface of things like bread or packaging often give rise to concern in the consumer’s mind because of a perceived health risk. Similarly,
the presence of material which looks like meat in a vegetarian product can give rise to complaints.
Identification of the plant structure from which a sample comes is based on the recognition of cell types present,
as seen under the compound microscope, and by comparison with published drawings, anatomical texts and known reference samples. The
likelihood of an identification will depend on many factors, including the size of the sample, which part of the plant the sample originates
from, the presence of any characteristic features, and the condition of the sample. Certain structures are more resistant to cooking and
processing than others and are more likely to be present in highly processed foods. For example, the cells and hairs from the bran layers of
wheat can still be seen even in a highly processed product, such as puffed wheat, whereas the structural features of other cells may be
completely destroyed during processing.
Insects and other invertebrates
Insect and invertebrate contamination is a recurring problem within the food industry as these types of foreign body
are often difficult to remove and hard to detect. They can also arouse strong feelings of revulsion in the consumer and in such cases it is
essential that the invertebrate species in question is accurately identified and the point at which contamination of the product occurred is
Identification to species level of the insect or invertebrate involved in the contamination issue will often provide
essential information such as the geographical distribution, lifecycle and food preferences of the insect / invertebrate. This information
can then be used to determine where in the food chain contamination of the product occurred.
The identification of insects and other invertebrates is often a specialised task reserved for an experienced
entomologist. There are many thousands of invertebrate species present in the UK, and on a worldwide scale the number of species is in the
millions. This large number of potential species may seem daunting at first but with the right knowledge and experience it is possible to
identify some of the more common groups and species of insects and other invertebrates associated with food products.
Microorganisms, particularly fungal and mould growths, can often give rise to foreign body complaints. Fungi (moulds
and yeasts) are probably the main causes. The principal feature identifying such material as fungal matter is that under the microscope it
is seen to be composed of many fine, branching, tube-like structures (hyphae).
In contrast to moulds, yeasts are fungi existing as groups of single cells (rather than branching structures) and
are identified as such by the presence of many round or oval cells, often seen “budding” or in chains. Yeasts are generally seen as a surface
film, smear or spots on the surface of a food product, or as a cloud or haze in liquid product, rather than as a solid body.
Despite the widespread use of metal detectors on production lines in the food industry, metal fragments still
represent a common source of foreign body complaints. Metal fragments may originate with the raw materials, they may derive from food
processing, or they may be introduced by the consumer. The accurate identification of the metal composition (e.g. by X-ray microanalysis) is
often an important factor in the correct identification of the source of the problem.
Metal fragments in food may be from a number of possible sources, such as tinplate from a can, stainless steel from
a factory pump or scraped surface heat exchanger, steel wool cleaning / scouring pads, chrome or nickel plating, or dental fillings from the
complainant themselves. Stainless steels are widely used in the food industry and so metal foreign bodies can often originate from food
Larger fragments of metal present few identification problems, as their metallic nature is usually readily apparent
from familiar physical characteristics. Clean metals or alloys such as copper, brass and bronze have characteristic colours. Careful
examination with a stereomicroscope will show evidence of wear, rusting or other surface changes.
The shape of the specimen is often of considerable value for identification. Fragments from saw blades, cutters and
stirrers have a characteristic shape. Wire has a characteristic cylindrical shape, whilst steel wool scouring pads may have a flattened,
Glass and ceramics
Glass fragments arising from consumer complaints are amongst the most significant foreign bodies that have to be
identified because of the public perception of glass as a particularly dangerous type of foreign body. It is therefore particularly important
to be able to identify glass fragments as accurately and as precisely as possible. Glass fragments may come from a wide variety of sources,
and it is important to bear all of these in mind when examining a sample. Most modern food factories operate a “no glass” policy in food
production areas, but there may still be glass inspection ports, dial covers, fluorescent lighting tubes and windows. On-line breakages,
particularly at the filler, are a potential hazard when food products are being packed in glass. However, we have found that many of the
glass fragments reported from food originate, whether by accident or design, from the consumer’s own home. A particularly common foreign
body example is a glass fragment from the bottom corner of a jar. These are produced when the last remaining contents of the jar are being
scraped out with a metal spoon or knife, and excess pressure causes a small hole to be made in the bottom corner of the jar.
Another frequently encountered glass fragment occurring as a result of accidental breakage in the home is a sliver
of glass from the rim of a heat resistant casserole dish or bowl. These pieces are usually sharply pointed at both ends and may be quite
Rocks and minerals
This group of foreign bodies ranges from grains of sand to larger stones. They commonly originate from the soil and
are harvested with a field crop. It is important to be able to recognise them for what they are, to avoid confusion with foreign bodies from
other sources, such as limescale. In many cases the item may be a very common material, such as sand, quartz or limestone. Nevertheless, this
may still be useful in narrowing down the range of possible origins for the sample.
Plastics have a wide range of uses in packaging, machinery and instrumentation, and in many domestic applications.
The wide range of uses means that they are amongst the most common foreign bodies to be reported. This is exacerbated by the fact that,
because of their physical characteristics, plastics are generally rather hard to detect within a food product, so it is often difficult to
remove such foreign bodies on-line.
In some cases, the shape of the piece of plastic can give an indication of its likely source. However, in many cases
the laboratory is asked to examine a small fragment that has broken off of a much larger object and, in cases like these, the shape of the
sample may not be particularly helpful.
Colour can sometimes be useful; the presence of bright or multiple colours can sometimes be an indication that one
is dealing with a consumer item rather than a piece of factory machinery, but this is not always the case. Different plastics are suitable
for different purposes, so the chemical identification of the plastic will often lead to a more limited range of possible / likely
Fibres and hairs
This group of foreign bodies includes six main classes of materials:
- Animal fibres (including human hairs)
- Insect fibres – silk and webbing (e.g. from caterpillars and spiders)
- Plant fibres and plant hairs, including cotton, linen, rape and sacking
- Artificial fibres (including synthetic, regenerated and glass fibres)
- Mineral fibres (asbestos, asbestos-like materials and carbon fibres)
Other than animal hairs, the majority of fibres encountered in foreign body identification work are from textiles,
filters, or rope and twine. They may be found as single strands, twisted or spun fibres or yarn, or as part of a structural material such as
cloth or paper.
The six main classes may be distinguished by their structure as seen under the light microscope and a range of
techniques can then be applied to identify the sample more precisely within one of these six main classes. Animal fibres, insect fibres and
plant fibres are usually identified by light microscopy; animal hairs may be foreign bodies in their own right, or may be present in droppings,
when they can be used to identify the animal responsible, such as rodents, rabbits and bats. Artificial fibres are often identified by
infra-red spectroscopy, whilst x-ray analysis can be useful for mineral and metal fibres.
The apparent contamination of food products with tablets or capsules is concerning for the consumer. Whilst the
material may be a prescribed medicine, it may equally be something as innocuous as an artificial sweetener tablet or a vitamin supplement.
Tablets and capsules may get into the food as a result of deliberate contamination or by accidental introduction, for instance by a preoccupied
or unwell consumer.
Tablets and capsules will often not survive most food processing operations. Most tablets and capsules are designed
to dissolve, so any prolonged contact with anything moist will often result in either complete dissolution or at least a very substantial
change in appearance. Consequently, it is unusual for contamination during the manufacturing operation to result in a physical contaminant
being detected by the consumer.
Naturally occurring materials reported as foreign bodies
A number of substances naturally present in food may be reported as foreign material. These may include items such
as fish bones in fish products, extraneous vegetable matter (EVM) in fruit and vegetables, indeterminate lumps of various kinds, and
crystalline materials of different types. Lumps of material may often contain carbohydrates (such as starch), proteins or lipids, and the
identification of these constituents can go a long way towards determining the cause of the problem.
Some of these materials may be described as ergastic substances (organic and inorganic end products of metabolism),
which do not participate in the building of the cell (e.g. gums, latex), and are found in the cytoplasm or in the vacuoles of cells. Reserve
food materials such as starch grains, aleurone grains and oils may be present in solution or as solid particles. Some of these, such as
starch, are important as food materials, and others are important either as foreign materials in their own right, or as evidence for the
identification of foreign bodies.
Crystals are often of particular concern, as they may be mistaken for glass fragments when found by the consumer.
They are formed as end products of metabolism, are of differing compositions, and are found in many kinds of plants. These can be seen in
the cells when examined microscopically. Calcium salts are the most common, especially calcium oxalate.
One particular crystal that is often mistaken for glass is struvite (magnesium ammonium phosphate), which is
occasionally found in fish products after canning. As a result, struvite can be reported in tuna sandwiches. Its presence is believed to be
related to a complex interaction of the nutritional components present in the product during processing, and the precise conditions of the
temperature and pressure being used. The crystals are formed in regular shaped prisms with straight edges, unlike glass, which fracture with
irregular edges. Struvite crystals are softer than glass, they can be scratched with a scalpel and also dissolve in warm solutions of dilute
vinegar or lemon juice (and more slowly in water). Whilst crystals suspected of being glass may be dissolved to show they are not glass, it
is important to identify the type of crystal present using its chemical / elemental composition.
Effects of processing on foreign bodies
When identifying foreign bodies and their origins, it is important to consider the potential effects of processing
on the object. Processing may change the appearance of the contaminant, and the nature of the change may suggest what type of processing it
has been subjected to. Our foreign body identification laboratory is frequently asked to determine whether a sample was processed with the
food product, in an attempt to determine at what point in the food chain the contamination occurred.
Alkaline phosphatase test
The alkaline phosphatase test has long been used as a presumptive indication as to whether or not an insect has been
heat-treated with a food product. This enzyme is present in virtually all living organisms, and remains active for a long period after death.
However, enzyme activity is destroyed by heat treatment. It is therefore assumed that alkaline phosphatase enzymes will remain active in an
insect long after its death, unless that insect is heat-treated; hence the test is widely used to indicate whether an insect has been
heat-treated. This test is not as 'exact' as it would at first appear, and careful interpretation of results is essential.
Effects of Chewing
Chewing of a foreign body by the complainant at the time of discovery may result in considerable damage, which may
make the task of identification that much more difficult. However, chewing may produce some characteristic teeth marks on more durable
materials such as plastic, which may help to substantiate the complainant’s account of events.
If a foreign body is received with a claim that it injured the complainant’s mouth, it may be possible to find
evidence of blood in some cases.
The range of items that occur in food as physical contaminants is wide. Identifying precisely what they are and how
they might have got into the food requires an array of techniques, and much knowledge and experience. Sometimes, all that is possible is to
eliminate some options. For example, an item could not have gained access at a certain point, or an item is not comprised of plastic, rubber,
metal or paint.
Some items reported as foreign bodies are, in fact, natural constituents of the food (although sometimes not
desirable natural constituents). It is important that companies treat all such complaints seriously, and have the nature of the contaminant
fully investigated when appropriate.
Get in touch for support
Our scientists have a wide range of expertise and analytical techniques at their disposal to provide you with the
data that you need when navigating foreign body challenges.
If you have a foreign body or other contamination issue in need of investigation, or you would like to find out more
about our microscopy or other services, please get in touch.
White paper download
Click below to download a copy of the white paper in PDF format
How can we help?
If you’d like to find out more about our foreign body analysis services, contact our support team
to find out how we can help.